Fluorescence imaging in the second near-infrared window (NIR-II, 900-1880 nm) offers high signal-to-background ratio (SBR), enhanced definition, and superior tissue penetration, making it ideal for real-time surgical navigation. However, with single-channel imaging, surgeons must frequently switch between the surgical field and the NIR-II images on the monitor. To address this issue, a coaxial dual-channel imaging system that combines visible light and 1100 nm long-pass (1100LP) fluorescence was developed. The system features a customized coaxial dual-channel lens with optimized distortion, achieving precise alignment with an error of less than ±0.15 mm. Additionally, the shared focusing mechanism simplifies operation. Using FDA-approved indocyanine green (ICG), the system was successfully applied in dual-channel guided rat lymph node excision, and blood supply assessment of reconstructed human flap. This approach enhances surgical precision, improves operational efficiency, and provides a valuable reference for further clinical translation of NIR-II fluorescence imaging.